A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed.
نویسندگان
چکیده
While often thought of as a smoking drug, tobacco (Nicotiana spp.) is now considered as a plant of choice for molecular farming and biofuel production. Here, we describe a noninvasive means of deriving both the distribution of lipid and the microtopology of the submillimeter tobacco seed, founded on nuclear magnetic resonance (NMR) technology. Our platform enables counting of seeds inside the intact tobacco capsule to measure seed sizes, to model the seed interior in three dimensions, to quantify the lipid content, and to visualize lipid gradients. Hundreds of seeds can be simultaneously imaged at an isotropic resolution of 25 µm, sufficient to assess each individual seed. The relative contributions of the embryo and the endosperm to both seed size and total lipid content could be assessed. The extension of the platform to a range of wild and cultivated Nicotiana species demonstrated certain evolutionary trends in both seed topology and pattern of lipid storage. The NMR analysis of transgenic tobacco plants with seed-specific ectopic expression of the plastidial phosphoenolpyruvate/phosphate translocator, displayed a trade off between seed size and oil concentration. The NMR-based assay of seed lipid content and topology has a number of potential applications, in particular providing a means to test and optimize transgenic strategies aimed at the manipulation of seed size, seed number, and lipid content in tobacco and other species with submillimeter seeds.
منابع مشابه
Breakthrough Technologies A Noninvasive Platform for Imaging and Quantifying Oil Storage in Submillimeter Tobacco Seed1[W][OA]
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, 06466 Gatersleben, Germany (J.F., H.R., S.S., A.B., L.B.); University of Würzburg, Institute of Experimental Physics 5, 97074 Wuerzburg, Germany (J.F., P.J.); The Huck Institutes of the Life Sciences and Department of Bioengineering, Pennsylvania State University, University Park, Pennsylvania 16802 (T.N.); Microbiologist (Atlant...
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ورودعنوان ژورنال:
- Plant physiology
دوره 161 2 شماره
صفحات -
تاریخ انتشار 2013